lightcycler 480 probes master reaction mix Search Results


lightcycler 480 probe master  (Roche)
99
Roche lightcycler 480 probe master
Lightcycler 480 Probe Master, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primer assay  (Quantitech)
90
Quantitech primer assay
Primer Assay, supplied by Quantitech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt pcr platform  (Revvity)
98
Revvity rt pcr platform
Rt Pcr Platform, supplied by Revvity, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt pcr platform - by Bioz Stars, 2026-07
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lightcycler 480 probes master mix  (Bio-Rad)
91
Bio-Rad lightcycler 480 probes master mix
Metabolic scheme for the production of [M1] (the active form of the drug) by esterase enzymes and [M5] by <t>CYP3A</t> enzymes.
Lightcycler 480 Probes Master Mix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lightcycler+480+probes+master+reaction+mix/pmc03869485-81-30-21?v=Bio-Rad
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lightcycler 480 probes master mix - by Bioz Stars, 2026-07
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lightcycler 480 probe master kit  (Nippon Genetics)
86
Nippon Genetics lightcycler 480 probe master kit
Metabolic scheme for the production of [M1] (the active form of the drug) by esterase enzymes and [M5] by <t>CYP3A</t> enzymes.
Lightcycler 480 Probe Master Kit, supplied by Nippon Genetics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lightcycler 480 probe master kit - by Bioz Stars, 2026-07
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circphact4 8  (Bio-Rad)
99
Bio-Rad circphact4 8
Metabolic scheme for the production of [M1] (the active form of the drug) by esterase enzymes and [M5] by <t>CYP3A</t> enzymes.
Circphact4 8, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp fgf10 rn00564115 m1  (Thermo Fisher)
86
Thermo Fisher gene exp fgf10 rn00564115 m1
Metabolic scheme for the production of [M1] (the active form of the drug) by esterase enzymes and [M5] by <t>CYP3A</t> enzymes.
Gene Exp Fgf10 Rn00564115 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lightcycler 480 probes masters  (TIB MOLBIOL)
90
TIB MOLBIOL lightcycler 480 probes masters
Metabolic scheme for the production of [M1] (the active form of the drug) by esterase enzymes and [M5] by <t>CYP3A</t> enzymes.
Lightcycler 480 Probes Masters, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lightcycler 480 probe master  (TIB MOLBIOL)
90
TIB MOLBIOL lightcycler 480 probe master
Metabolic scheme for the production of [M1] (the active form of the drug) by esterase enzymes and [M5] by <t>CYP3A</t> enzymes.
Lightcycler 480 Probe Master, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Metabolic scheme for the production of [M1] (the active form of the drug) by esterase enzymes and [M5] by CYP3A enzymes.

Journal: F1000Research

Article Title: Regulation of CYP3A genes by glucocorticoids in human lung cells

doi: 10.12688/f1000research.2-173.v2

Figure Lengend Snippet: Metabolic scheme for the production of [M1] (the active form of the drug) by esterase enzymes and [M5] by CYP3A enzymes.

Article Snippet: Total RNA was isolated from cells using TRIzol reagent (Life Technologies). cDNA was synthesized using iScript Reverse Transcription Supermix for qPCR (BIO RAD, Hercules, CA). qPCR was performed using either LightCycler 480 Probes Master mix (CYP3A5*1) or LightCycler 480 SYBR Green I Master Mix (all other genes) (Roche, Indianapolis, IN) with a LightCycler 480 System.

Techniques:

( A ) Active glucocorticoid will bind to the glucocorticoid receptor (GR), which forms a homodimer and translocates to the nucleus. The homodimer binds to its response element (GRE) and induces the expression of either the pregnane X receptor (PXR) or the constitutive androstane receptor (CAR). CAR or PXR (though this receptor is not expressed in the lungs) forms a heterodimer with the retinoic X receptor alpha (RXRα) which in turn induces the expression of the CYP3A enzymes via binding of the respective response-elements (CARE and/or PXRE). ( B ) Active glucocorticoid will bind to the glucocorticoid receptor (GR), which forms a homodimer and translocates into the nucleus. The homodimer binds to its response element (GRE) and induces the expression of CYP3A enzymes.

Journal: F1000Research

Article Title: Regulation of CYP3A genes by glucocorticoids in human lung cells

doi: 10.12688/f1000research.2-173.v2

Figure Lengend Snippet: ( A ) Active glucocorticoid will bind to the glucocorticoid receptor (GR), which forms a homodimer and translocates to the nucleus. The homodimer binds to its response element (GRE) and induces the expression of either the pregnane X receptor (PXR) or the constitutive androstane receptor (CAR). CAR or PXR (though this receptor is not expressed in the lungs) forms a heterodimer with the retinoic X receptor alpha (RXRα) which in turn induces the expression of the CYP3A enzymes via binding of the respective response-elements (CARE and/or PXRE). ( B ) Active glucocorticoid will bind to the glucocorticoid receptor (GR), which forms a homodimer and translocates into the nucleus. The homodimer binds to its response element (GRE) and induces the expression of CYP3A enzymes.

Article Snippet: Total RNA was isolated from cells using TRIzol reagent (Life Technologies). cDNA was synthesized using iScript Reverse Transcription Supermix for qPCR (BIO RAD, Hercules, CA). qPCR was performed using either LightCycler 480 Probes Master mix (CYP3A5*1) or LightCycler 480 SYBR Green I Master Mix (all other genes) (Roche, Indianapolis, IN) with a LightCycler 480 System.

Techniques: Expressing, Binding Assay

Primer sequences for qPCR assays.

Journal: F1000Research

Article Title: Regulation of CYP3A genes by glucocorticoids in human lung cells

doi: 10.12688/f1000research.2-173.v2

Figure Lengend Snippet: Primer sequences for qPCR assays.

Article Snippet: Total RNA was isolated from cells using TRIzol reagent (Life Technologies). cDNA was synthesized using iScript Reverse Transcription Supermix for qPCR (BIO RAD, Hercules, CA). qPCR was performed using either LightCycler 480 Probes Master mix (CYP3A5*1) or LightCycler 480 SYBR Green I Master Mix (all other genes) (Roche, Indianapolis, IN) with a LightCycler 480 System.

Techniques:

( A ) Relative quantities of [M1] and [M5] measured by LC/MS/MS in A549 cell culture media following beclomethasone dipropionate (BDP) treatment alone, BDP + esterase inhibitors (EI), 1-aminobenzotriazole (1-ABT) + BDP, and 1-ABT + EI + [M1]. ( B ) CYP3A5 mRNA detected in A549 cells following DMSO control, BDP treatment alone, BDP + EI, 1-ABT alone, 1-ABT + BDP, and 1-ABT + EI + [M1]. Values are expressed as fold over DMSO controls. Statistics used for data analysis were one-way ANOVA with Dunnett’s post-hoc test. Data are the mean and standard deviation from n=6 replicates. * p<0.05, ** p<0.01.

Journal: F1000Research

Article Title: Regulation of CYP3A genes by glucocorticoids in human lung cells

doi: 10.12688/f1000research.2-173.v2

Figure Lengend Snippet: ( A ) Relative quantities of [M1] and [M5] measured by LC/MS/MS in A549 cell culture media following beclomethasone dipropionate (BDP) treatment alone, BDP + esterase inhibitors (EI), 1-aminobenzotriazole (1-ABT) + BDP, and 1-ABT + EI + [M1]. ( B ) CYP3A5 mRNA detected in A549 cells following DMSO control, BDP treatment alone, BDP + EI, 1-ABT alone, 1-ABT + BDP, and 1-ABT + EI + [M1]. Values are expressed as fold over DMSO controls. Statistics used for data analysis were one-way ANOVA with Dunnett’s post-hoc test. Data are the mean and standard deviation from n=6 replicates. * p<0.05, ** p<0.01.

Article Snippet: Total RNA was isolated from cells using TRIzol reagent (Life Technologies). cDNA was synthesized using iScript Reverse Transcription Supermix for qPCR (BIO RAD, Hercules, CA). qPCR was performed using either LightCycler 480 Probes Master mix (CYP3A5*1) or LightCycler 480 SYBR Green I Master Mix (all other genes) (Roche, Indianapolis, IN) with a LightCycler 480 System.

Techniques: Liquid Chromatography with Mass Spectroscopy, Cell Culture, Standard Deviation

Comparison of glucocorticoid receptor (GR), constitutive androstane receptor (CAR), and CYP3A5 mRNA expression in lung cell cultures.

Journal: F1000Research

Article Title: Regulation of CYP3A genes by glucocorticoids in human lung cells

doi: 10.12688/f1000research.2-173.v2

Figure Lengend Snippet: Comparison of glucocorticoid receptor (GR), constitutive androstane receptor (CAR), and CYP3A5 mRNA expression in lung cell cultures.

Article Snippet: Total RNA was isolated from cells using TRIzol reagent (Life Technologies). cDNA was synthesized using iScript Reverse Transcription Supermix for qPCR (BIO RAD, Hercules, CA). qPCR was performed using either LightCycler 480 Probes Master mix (CYP3A5*1) or LightCycler 480 SYBR Green I Master Mix (all other genes) (Roche, Indianapolis, IN) with a LightCycler 480 System.

Techniques: Expressing

CYP3A5 mRNA detected in A549 cells treated with ( A ) beclomethasone dipropionate (BDP), ( B ) budesonide (BUD), ( C ) triamcinolone acetonide (TCL), ( D ) fluticasone propionate (FLT), and ( E ) flunisolide (FLN), with and without ketoconazole (KTZ), a competitive antagonist for GR. Results are presented as fold over DMSO controls. Statistics used for data analysis were one-way ANOVA with Dunnett’s post-hoc test. Data are the mean and standard deviation from n=6 replicates. * p<0.05, ** p<0.01, *** p<0.001.

Journal: F1000Research

Article Title: Regulation of CYP3A genes by glucocorticoids in human lung cells

doi: 10.12688/f1000research.2-173.v2

Figure Lengend Snippet: CYP3A5 mRNA detected in A549 cells treated with ( A ) beclomethasone dipropionate (BDP), ( B ) budesonide (BUD), ( C ) triamcinolone acetonide (TCL), ( D ) fluticasone propionate (FLT), and ( E ) flunisolide (FLN), with and without ketoconazole (KTZ), a competitive antagonist for GR. Results are presented as fold over DMSO controls. Statistics used for data analysis were one-way ANOVA with Dunnett’s post-hoc test. Data are the mean and standard deviation from n=6 replicates. * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: Total RNA was isolated from cells using TRIzol reagent (Life Technologies). cDNA was synthesized using iScript Reverse Transcription Supermix for qPCR (BIO RAD, Hercules, CA). qPCR was performed using either LightCycler 480 Probes Master mix (CYP3A5*1) or LightCycler 480 SYBR Green I Master Mix (all other genes) (Roche, Indianapolis, IN) with a LightCycler 480 System.

Techniques: Standard Deviation

Modifications made to SAEC culture media.

Journal: F1000Research

Article Title: Regulation of CYP3A genes by glucocorticoids in human lung cells

doi: 10.12688/f1000research.2-173.v2

Figure Lengend Snippet: Modifications made to SAEC culture media.

Article Snippet: Total RNA was isolated from cells using TRIzol reagent (Life Technologies). cDNA was synthesized using iScript Reverse Transcription Supermix for qPCR (BIO RAD, Hercules, CA). qPCR was performed using either LightCycler 480 Probes Master mix (CYP3A5*1) or LightCycler 480 SYBR Green I Master Mix (all other genes) (Roche, Indianapolis, IN) with a LightCycler 480 System.

Techniques: Cell Culture, Expressing